Chromosomal Bands Affected by Acute Oil Exposure and DNA Repair Errors

Chromosomal Bands Affected by Acute Oil Exposure and DNA Repair Errors

BACKGROUND In a previous study, we showed that individuals who had participated in oil clean-up tasks after the wreckage of the Prestige presented an increase of structural chromosomal alterations two years after the acute exposure had occurred. Other studies have also reported the presence of DNA damage during acute oil exposure, but little is known about the long term persistence of chromosom...

Nickel Increases Chromosomal Abnormalities by Interfering with the Initiation of DNA Repair Pathways

Background: Nickel is a carcinogenic, heavy metal released through industrial activities and via natural resources. It is able to cause DNA damages by reducing the efficiency of DNA repair mechanisms. However, the exact time point at which it is able to interfere with these mechanisms is not yet clearly understood. Methods: To find the most nickel-vulnerable time of repair mechanisms, human de...

Persistence of Breakage in Specific Chromosome Bands 6 Years after Acute Exposure to Oil

BACKGROUND The identification of breakpoints involved in chromosomal damage could help to detect genes involved in genetic disorders, most notably cancer. Until now, only one published study, carried out by our group, has identified chromosome bands affected by exposure to oil from an oil spill. In that study, which was performed two years after the initial oil exposure in individuals who had p...

Suppression of gene amplification and chromosomal DNA integration by the DNA mismatch repair system.

Mismatch repair (MMR)-deficient cells are shown to produce >15-fold more methotrexate-resistant colonies than MMR normal cells. The increased resistance to methotrexate is primarily due to gene amplification since all the resistant clones contain double-minute chromosomes and increased copy numbers of the DHFR gene. In addition, integration of linearized or retroviral DNAs into chromosomes is a...

Characterization of Avian Pasteurella multocida Isolates by Protein Profiles and Restriction Enzyme Analysis of Chromosomal DNA